nb100 (Novus Biologicals)
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Novus Biologicals
nb100
Nb100, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rpe65/RPE65+Antibody+(401%2E8B11%2E3D9)+-+BSA+Free/pmc12995699-8-7-3
Average 95 stars, based on 45 article reviews
Nb100, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rpe65/RPE65+Antibody+(401%2E8B11%2E3D9)+-+BSA+Free/pmc12995699-8-7-3
Average 95 stars, based on 45 article reviews
nb100 - by Bioz Stars,
2026-10
95/100 stars
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Incubation:Article Title: CRX is an intrinsic suppressor of epithelial‒mesenchymal transition in retinal pigment epithelial cells: a promising therapeutic avenue for subretinal fibrosis. Article Snippet: For immunostaining, fixed cells and cryosections from eyes were permeabilized with 0.25% Triton X-100 (Sigma) for 2 min, washed with PBS, and then blocked with 2% bovine serum albumin (BSA, Sigma) in PBS. .. The samples were incubated with the primary antibodies against α-SMA (Abcam, Cambridge, UK), ZO-1 (Proteintech, Chicago, USA), CRX (Proteintech), YAP1 (Proteintech), β-catenin (Proteintech), and Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration Article Snippet: For immunofluorescence analysis, fixed cells, cryosections from eyes and matrigel containing transplanted cells in nude mice, were permeabilized with 0.25% Triton X-100 (Sigma) for 2 min, washed with PBS, and then blocked with 2% bovine serum albumin (BSA, Sigma) in PBS. .. The sections were incubated with the primary antibodies (1:200) against OCT4, NANOG, SOX2, ZO-1, FN1, LIN7A, PARD6B, CRALBP, BEST1, PPM1A, MITF-A, CRX and C-MYC (Proteintech, Rosemont, IL); against SSEA4, TYRP1, α-SMA, FOXF2, MYOSIN, p -SMAD2, p -SMAD3, NR2E1, Rhodopsin, and Ki67 (Abcam, Cambridge, UK); against Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration. Article Snippet: For immunofluorescence analysis, fixed cells, cryosections from eyes and matrigel containing transplanted cells in nude mice, were permeabilized with 0.25% Triton X-100 (Sigma) for 2 min, washed with PBS, and then blocked with 2% bovine serum albumin (BSA, Sigma) in PBS. .. The sections were incubated with the primary antibodies (1:200) against OCT4, NANOG, SOX2, ZO-1, FN1, LIN7A, PARD6B, CRALBP, BEST1, PPM1A, MITF-A, CRX and C-MYC (Proteintech, Rosemont, IL); against SSEA4, TYRP1, a-SMA, FOXF2, MYOSIN, p-SMAD2, p-SMAD3, NR2E1, Rhodopsin, and Ki67 (Abcam, Cambridge, UK); against Article Title: Targeting senescent retinal pigment epithelial cells facilitates retinal regeneration in mouse models of age-related macular degeneration Article Snippet: .. After blocking with 1% BSA in PBS for 1 h, fixed tissues or cells were incubated overnight at 4 °C with primary antibodies against BrdU (1:1000, Invitrogen, MA3-071, CA, USA), p53 (1:50, Santa Cruz Biotechnology, TX, USA), p21 (1:50, Santa Cruz Biotechnology), p16 (1:100, BD Bioscience), Ki67 (1:1000, Abcam, Cambridge, UK), HMGB1 (1:1000, Abcam, ab18256), H3K9me3 (1:1000, Abcam, ab8898), γ-H2AX (1:1000, Abcam, ab2893), ZO-1 (1:250, Invitrogen, 61–7300, 33–9100), APOE (1:50, Santa Cruz Biotechnology), Article Title: CRX is an intrinsic suppressor of epithelial‒mesenchymal transition in retinal pigment epithelial cells: a promising therapeutic avenue for subretinal fibrosis Article Snippet: For immunostaining, fixed cells and cryosections from eyes were permeabilized with 0.25% Triton X-100 (Sigma) for 2 min, washed with PBS, and then blocked with 2% bovine serum albumin (BSA, Sigma) in PBS. .. The samples were incubated with the primary antibodies against α-SMA (Abcam, Cambridge, UK), ZO-1 (Proteintech, Chicago, USA), CRX (Proteintech), YAP1 (Proteintech), β-catenin (Proteintech), and Immunofluorescence:Article Title: Tenascin-C induces transdifferentiation of retinal pigment epithelial cells in proliferative vitreoretinopathy. Article Snippet: 37 Proliferation and transdifferentiation of the retinal pigment epithelium (RPE) are 38 hallmarks of proliferative vitreoretinopathy (PVR); however, the critical regulators of 39 this process remain to be elucidated.. Here, we investigated the role of tenascin-C in 40 PVR development.. In vitro, exposure of human ARPE-19 (hRPE) cells to TGF-β2 41 increased tenascin-C expression. Expressing:Article Title: Tenascin-C induces transdifferentiation of retinal pigment epithelial cells in proliferative vitreoretinopathy. Article Snippet: 37 Proliferation and transdifferentiation of the retinal pigment epithelium (RPE) are 38 hallmarks of proliferative vitreoretinopathy (PVR); however, the critical regulators of 39 this process remain to be elucidated.. Here, we investigated the role of tenascin-C in 40 PVR development.. In vitro, exposure of human ARPE-19 (hRPE) cells to TGF-β2 41 increased tenascin-C expression. Western Blot:Article Title: Evaluating precision medicine approaches for gene therapy in patient-specific cellular models of Bietti crystalline dystrophy Article Snippet: .. Primary antibodies against the following proteins were used for Western blots: CYP4V2 (rabbit polyclonal, 1:1500, MilliporeSigma, SAB1410565); Blocking Assay:Article Title: Targeting senescent retinal pigment epithelial cells facilitates retinal regeneration in mouse models of age-related macular degeneration Article Snippet: .. After blocking with 1% BSA in PBS for 1 h, fixed tissues or cells were incubated overnight at 4 °C with primary antibodies against BrdU (1:1000, Invitrogen, MA3-071, CA, USA), p53 (1:50, Santa Cruz Biotechnology, TX, USA), p21 (1:50, Santa Cruz Biotechnology), p16 (1:100, BD Bioscience), Ki67 (1:1000, Abcam, Cambridge, UK), HMGB1 (1:1000, Abcam, ab18256), H3K9me3 (1:1000, Abcam, ab8898), γ-H2AX (1:1000, Abcam, ab2893), ZO-1 (1:250, Invitrogen, 61–7300, 33–9100), APOE (1:50, Santa Cruz Biotechnology), |
